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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UCH-L1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.781-100 ng/mL

Sandwich ELISA: 0.625-20 ng/mL

Cat No : MP01079-2

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01079-2, Human UCHL1 Recombinant Matched Antibody Pair - PBS only. 84154-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6490. 84154-3-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL

    Sandwich ELISA standard curve of MP01079-2

    Sandwich ELISA standard curve of MP01079-2, Human UCHL1 Recombinant Matched Antibody Pair - PBS only. 84154-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6490. 84154-3-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL

  • Serum of ten individual healthy human donors was measured. The human UCHL1 concentration of detected samples was determined to be 8.99 ng/mL with a range of 0.13 - 110.20 ng/mL.

    Sample test of MP01079-2

    Serum of ten individual healthy human donors was measured. The human UCHL1 concentration of detected samples was determined to be 8.99 ng/mL with a range of 0.13 - 110.20 ng/mL.

  • Cytometric bead array standard curve of MP01079-2, UCHL1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84154-2-PBS. Detection antibody: 84154-3-PBS. Standard: Ag6490. Range: 0.781-100 ng/mL.

    Cytometric bead array standard curve of MP01079-2

    Cytometric bead array standard curve of MP01079-2, UCHL1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84154-2-PBS. Detection antibody: 84154-3-PBS. Standard: Ag6490. Range: 0.781-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01079-2 targets UCH-L1 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.781-100 ng/mL (Cytometric Bead Array); 0.625-20 ng/mL (Sandwich ELISA);

Capture antibody: UCH-L1/PGP9.5 Recombinant antibody, PBS Only (Capture) 84154-2-PBS (241107C5). 100 μg. Concentration 1 mgl/ml.

Detection antibody: UCH-L1/PGP9.5 Recombinant antibody, PBS Only (Capture/Detector) 84154-3-PBS (241107G1). 100 μg. Concentration 1 mgl/ml.

Alternative UCH-L1 matched antibody pairs: MP01079-1, MP01079-3, MP50875-1

Matched pair protein standard: Ag6490

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84154-2-PBS
Tested Reactivity human
Immunogen UCH-L1/PGP9.5 fusion protein Ag6490
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Synonyms PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5
GenBank accession numberBC000332
Gene symbol UCHL1
UniprotP09936
Gene ID (NCBI) 7345
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84154-3-PBS
Tested Reactivity human
Immunogen UCH-L1/PGP9.5 fusion protein Ag6490
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Synonyms PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5
GenBank accession numberBC000332
Gene symbol UCHL1
UniprotP09936
Gene ID (NCBI) 7345
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.