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  • Featured Product
  • KD/KO Validated

UCHL5 Polyclonal antibody, PBS Only

UCHL5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11527-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using UCHL5 Polyclonal antibody (11527-1-AP)

    WB analysis of mouse colon using 11527-1-AP (same clone as 11527-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 11527-1-AP (UCHL5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using UCHL5 Polyclonal antibody (11527-1-AP)

    WB analysis of mouse brain using 11527-1-AP (same clone as 11527-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11527-1-AP (UCHL5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using UCHL5 Polyclonal antibody (11527-1-AP)

    IHC staining of mouse kidney using 11527-1-AP (same clone as 11527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11527-1-AP (UCHL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using UCHL5 Polyclonal antibody (11527-1-AP)

    IHC staining of mouse kidney using 11527-1-AP (same clone as 11527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11527-1-AP (UCHL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11527-1-PBS targets UCHL5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCHL5 fusion protein Ag2095 相同性解析による交差性が予測される生物種
Full Name ubiquitin carboxyl-terminal hydrolase L5
Calculated molecular weight 326 aa, 37 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC025369
Gene symbol UCHL5
Gene ID (NCBI) 51377
RRIDAB_2877774
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UCHL5 (Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It is functionally linked with multiple protein complexes and signal transduction pathways. UCH37's weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of UCH37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given UCH37's monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for UCH37 monomers (PMID:21953935).