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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UCHL5 Monoclonal antibody (68535-1-Ig)

    WB analysis using 68535-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68535-1-Ig (UCHL5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HCT 116 cells using UCHL5 Monoclonal antibody (68535-1-Ig)

    WB analysis of HCT 116 using 68535-1-Ig

    WB result of UCHL5 antibody (68535-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UCHL5 transfected HCT 116 cells.

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  • KD/KO Validated

UCHL5 Monoclonal antibody

UCHL5 Monoclonal Antibody for WB, ELISA
Cat No. 68535-1-Ig
Clone No.2B8G11

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Synonyms

CGI 70, INO80R, Ubiquitin thioesterase L5, UCH L5, UCH37, UCHL5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, LNCaP cells, MCF-7 cells, HeLa cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68535-1-Ig targets UCHL5 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name ubiquitin carboxyl-terminal hydrolase L5
Calculated molecular weight 326 aa, 37 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC025369
Gene Symbol UCHL5
Gene ID (NCBI) 51377
RRIDAB_3085243
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y5K5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UCHL5 (Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It is functionally linked with multiple protein complexes and signal transduction pathways. UCH37's weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of UCH37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given UCH37's monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for UCH37 monomers (PMID:21953935).

Protocols

Product Specific Protocols
WB protocol for UCHL5 antibody 68535-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols