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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using UCHL5IP Polyclonal antibody (17830-1-AP)

    WB analysis of MCF-7 using 17830-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 17830-1-AP (UCHL5IP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using UCHL5IP Polyclonal antibody (17830-1-AP)

    IHC staining of human ovary using 17830-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 17830-1-AP (UCHL5IP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using UCHL5IP Polyclonal antibody (17830-1-AP)

    IHC staining of human ovary using 17830-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 17830-1-AP (UCHL5IP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using UCHL5IP Polyclonal antibody (17830-1-AP)

    IF Staining of MCF-7 using 17830-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 17830-1-AP (UCHL5IP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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UCHL5IP Polyclonal antibody

UCHL5IP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17830-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UIP1, UCHL5-interacting protein, UCHL5 interacting protein, HAUS7, HAUS augmin-like complex subunit 7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17830-1-AP targets UCHL5IP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCHL5IP fusion protein Ag12225 相同性解析による交差性が予測される生物種
Full Name UCHL5 interacting protein
Calculated molecular weight 368 aa, 41 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC008141
Gene Symbol UCHL5IP
Gene ID (NCBI) 55559
RRIDAB_2878447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99871
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UCHL5IP antibody 17830-1-APDownload protocol
IHC protocol for UCHL5IP antibody 17830-1-APDownload protocol
IF protocol for UCHL5IP antibody 17830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article