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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UCHL5IP Polyclonal antibody

UCHL5IP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17830-1-AP

Synonyms

UIP1, UCHL5-interacting protein, UCHL5 interacting protein, HAUS7, HAUS augmin-like complex subunit 7

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using UCHL5IP Polyclonal antibody (17830-1-AP)

    WB analysis of MCF-7 using 17830-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 17830-1-AP (UCHL5IP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using UCHL5IP Polyclonal antibody (17830-1-AP)

    IHC staining of human ovary using 17830-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 17830-1-AP (UCHL5IP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using UCHL5IP Polyclonal antibody (17830-1-AP)

    IHC staining of human ovary using 17830-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 17830-1-AP (UCHL5IP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using UCHL5IP Polyclonal antibody (17830-1-AP)

    IF Staining of MCF-7 using 17830-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 17830-1-AP (UCHL5IP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17830-1-AP targets UCHL5IP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCHL5IP fusion protein Ag12225 相同性解析による交差性が予測される生物種
Full Name UCHL5 interacting protein
Calculated molecular weight 368 aa, 41 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC008141
Gene symbol UCHL5IP
Gene ID (NCBI) 55559
RRIDAB_2878447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UCHL5IP antibody 17830-1-APDownload protocol
IHC protocol for UCHL5IP antibody 17830-1-APDownload protocol
IF protocol for UCHL5IP antibody 17830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article