Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
12271-1-AP targets UCK1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UCK1 fusion protein Ag2912 相同性解析による交差性が予測される生物種 |
| Full Name | uridine-cytidine kinase 1 |
| Calculated molecular weight | 277 aa, 31 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC015547 |
| Gene Symbol | UCK1 |
| Gene ID (NCBI) | 83549 |
| RRID | AB_11182173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HA47 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Uridine/cytidine kinase-1 is a pyrimidine ribonucleoside kinase that catalyzes the phosphorylation of uridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP). The deduced 277-amino acid protein has a calculated molecular mass of 31 kDa. UCK1 belongs to the uridine kinase family and UCK have important roles for the phosphorylation of nucleoside analogs that may be important in chemotherapy of cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UCK1 antibody 12271-1-AP | Download protocol |
| IF protocol for UCK1 antibody 12271-1-AP | Download protocol |
| IP protocol for UCK1 antibody 12271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Cancer Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism. | ||
Blood Adv Reprogramming of pyrimidine nucleotide metabolism supports vigorous cell proliferation of normal and malignant T cells | ||
Nat Metab Glucose limitation protects cancer cells from apoptosis induced by pyrimidine restriction and replication inhibition | ||
Oncogene The protection of UCK2 protein stability by GART maintains pyrimidine salvage synthesis for HCC growth under glucose limitation |