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- KD/KO Validated
UCK2 Polyclonal antibody
UCK2 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 10511-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 7 publications below |
| WB | See 16 publications below |
| IHC | See 2 publications below |
Product Information
10511-1-AP targets UCK2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UCK2 fusion protein Ag0782 相同性解析による交差性が予測される生物種 |
| Full Name | uridine-cytidine kinase 2 |
| Calculated molecular weight | 29 kDa |
| Observed molecular weight | 33-35 kDa |
| GenBank accession number | BC002906 |
| Gene symbol | UCK2 |
| Gene ID (NCBI) | 7371 |
| RRID | AB_2241182 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Uridine-cytidine kinase 2 catalyzes the phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP), respectively. This is the first step in the production of the pyrimidine nucleoside triphosphates required for RNA and DNA synthesis. UCK2 belongs to the uridine kinase family and UCK have important roles for the phosphorylation of nucleoside analogs that may be important in chemotherapy of cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UCK2 antibody 10511-1-AP | Download protocol |
| IP protocol for UCK2 antibody 10511-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Prolif Prognostic and predictive role of a metabolic rate-limiting enzyme signature in hepatocellular carcinoma.
| ||
Int J Cancer Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism. | ||
Pharmacol Res Perspect Lack of cross-resistance to FF-10501, an inhibitor of inosine-5'-monophosphate dehydrogenase, in azacitidine-resistant cell lines selected from SKM-1 and MOLM-13 leukemia cell lines. | ||
Cell Rep A reversible metabolic stress-sensitive regulation of CRMP2A orchestrates EMT/stemness and increases metastatic potential in cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Dan (Verified Customer) (05-27-2021) | Very cost effective
|
FH H (Verified Customer) (04-05-2020) | Primary antibody: 1:1000 dilution in 5% BSA, overnight incubation at 4CSecondary antibody: anti-rabbit IRDye800Detection: Odyssey ClxThis antibody works quite well for HEK293T and HCT116 cell lysates
 |
