Validation Data Gallery

Filter: AllWBIHC

WB analysis of K-562 using 17005-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 17005-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 17005-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 17005-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of mouse kidney using 17005-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17005-1-AP (UCKL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 17005-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17005-1-AP (UCKL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 17005-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 17005-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 17005-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 17005-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 17005-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 17005-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 17005-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 17005-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).

UCKL1 Polyclonal antibody

UCKL1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17005-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

F538, UCK1 LIKE, UCK1L, UCKL1, Uridine cytidine kinase like 1, URKL1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, Raji cells, rat kidney tissue, U-937 cells
Positive IHC detected in	mouse kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IHC	See 1 publications below

Product Information

17005-1-AP targets UCKL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UCKL1 fusion protein Ag10701 相同性解析による交差性が予測される生物種
Full Name	uridine-cytidine kinase 1-like 1
Calculated molecular weight	419 aa, 46 kDa
Observed molecular weight	61 kDa, 46 kDa
GenBank accession number	BC033078
Gene Symbol	UCKL1
Gene ID (NCBI)	54963
RRID	AB_2210783
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NWZ5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for UCKL1 antibody 17005-1-AP	Download protocol
IHC protocol for UCKL1 antibody 17005-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IHC	EBioMedicine Non-canonical role of UCKL1 on ferroptosis defence in colorectal cancer Authors - Weili Wu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of K-562 using 17005-1-AP

WB analysis of Raji using 17005-1-AP

WB analysis of U-937 using 17005-1-AP

WB analysis of rat kidney using 17005-1-AP

IHC staining of mouse kidney using 17005-1-AP