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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Urocortin Recombinant antibody, PBS Only

Urocortin Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241183B12

Cat no : 84380-1-PBS

Synonyms

UCN, UROC, UI, 241183B12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Urocortin Recombinant antibody (84380-1-RR)

    WB analysis using 84380-1-RR (same clone as 84380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84380-1-RR (UCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Urocortin Recombinant antibody (84380-1-RR)

    WB analysis using 84380-1-RR (same clone as 84380-1-PBS)

    Rat kidney tissue were subjected to SDS PAGE followed by western blot with 84380-1-RR (UCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Urocortin Recombinant antibody (84380-1-RR)

    IHC staining of mouse heart using 84380-1-RR (same clone as 84380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84380-1-RR (Urocortin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Urocortin Recombinant antibody (84380-1-RR)

    IHC staining of mouse heart using 84380-1-RR (same clone as 84380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84380-1-RR (Urocortin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84380-1-PBS targets Urocortin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name urocortin
Calculated molecular weight 13 kDa
Observed molecular weight22 kDa
GenBank accession numberNM_003353
Gene symbol UCN
Gene ID (NCBI) 7349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family that includes CRF, urotensin I, sauvagine, urocortin II and urocortin III. Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow. Urocortin acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) and binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin is synthesized as precursor, which is subsequently processed to the mature biologically active peptide (a 40-amino acid Molecule ). The 22 kDa may be a urocortin 1 precursor protein (PMID: 28887029).