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  • KD/KO Validated

UCP1 Polyclonal antibody

UCP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23673-1-AP

Synonyms

UCP 1, UCP, Thermogenin, SLC25A7


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Tested Applications

Positive WB detected inmouse brown adipose tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse brown adipose tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23673-1-AP targets UCP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, hamster, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCP1 fusion protein Ag20388 相同性解析による交差性が予測される生物種
Full Name uncoupling protein 1 (mitochondrial, proton carrier)
Calculated molecular weight 307 aa, 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC098168
Gene symbol UCP1
Gene ID (NCBI) 7350
RRIDAB_2828003
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.

Protocols

Product Specific Protocols
WB protocol for UCP1 antibody 23673-1-APDownload protocol
IHC protocol for UCP1 antibody 23673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu
  • KO Validated
mouseWB

Nat Commun

Improvement of obesity-associated disorders by a small-molecule drug targeting mitochondria of adipose tissue macrophages.

Authors - Yawei Wang
mouseWB,IHC

Mol Biol Evol

Functional Attenuation of UCP1 as the Potential Mechanism for Thickened Blubber Layer in Cetaceans

Authors - Ming Zhou
mouseWB

Diabetes

Exosomes From Adipose-Derived Stem Cells Attenuate Adipose Inflammation and Obesity Through Polarizing M2 Macrophages and Beiging in White Adipose Tissues.

Authors - Hui Zhao
bovineIF

Cell Prolif

Procyanidin B2 improves developmental capacity of bovine oocytes via promoting PPARγ/UCP1-mediated uncoupling lipid catabolism during in vitro maturation

Authors - Yuwen Luo
mouseWB

JCI Insight

Muscle specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism

Authors - Benedict Abdon

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mi (Verified Customer) (03-06-2023)

It works well in human brown adipocyte cells. Although we got several non-specific bands, the band at the expected size was very clear.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human brown adipocytes