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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using UCP5 Polyclonal antibody (25223-1-AP)

    WB analysis of fetal human brain using 25223-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25223-1-AP (UCP5 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using UCP5 Polyclonal antibody (25223-1-AP)

    WB analysis of PC-3 using 25223-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25223-1-AP (UCP5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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UCP5 Polyclonal antibody

UCP5 Polyclonal Antibody for WB, ELISA
Cat No. 25223-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

BMCP 1, BMCP1, SLC25A14, UCP 5, UCP5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected infetal human brain tissue, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25223-1-AP targets UCP5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCP5 fusion protein Ag18661 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier, brain), member 14
Calculated molecular weight 325 aa, 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC119666
Gene Symbol UCP5
Gene ID (NCBI) 9016
RRIDAB_2879971
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95258
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UCP5, also known as BMCP1 (brain mitochondrial carrier protein-1), is a member of uncoupling proteins (UCPs) that catalyze proton leaks across the inner mitochondrial membrane, thus uncoupling fuel oxidation from ATP synthesis. UCPs are implicated in metabolic rate and adaptational thermoregulation. UCP5 is widely expressed with high abundance in brain and testis. Alternative splicing generates several isoforms of UCP5.

Protocols

Product Specific Protocols
WB protocol for UCP5 antibody 25223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols