Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
15783-1-AP targets UFC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UFC1 fusion protein Ag8480 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin-fold modifier conjugating enzyme 1 |
| Calculated molecular weight | 167 aa, 19 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC005187 |
| Gene Symbol | UFC1 |
| Gene ID (NCBI) | 51506 |
| RRID | AB_2213938 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3C8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The function of UFC1 (ubiquitin fold modifier conjugating enzyme 1) has not been widely studied, and is yet to be fully elucidated. The MW of this protein is 21 kDa, and this antibody specially recognises the 21 kDa protein. Catalog#15783-1-AP specially recognises the 21 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UFC1 antibody 15783-1-AP | Download protocol |
| IHC protocol for UFC1 antibody 15783-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation | ||
Mol Cell UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance anti-tumor immunity
| ||
Cancer Lett CDK5RAP3 is a novel super-enhancer-driven gene activated by master TFs and regulates ER-Phagy in neuroblastoma | ||
BMC Cardiovasc Disord Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice |