Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of K-562 using 10615-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10615-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10615-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 10615-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 10615-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 10615-1-AP
IP result of anti-UFD1L (IP:10615-1-AP, 4ug; Detection:10615-1-AP 1:1000) with Raji cells lysate 2000ug.
IHC staining of human stomach using 10615-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10615-1-AP (UFD1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10615-1-AP
Immunofluorescent analysis of HeLa cells using 10615-1-AP (UFD1L antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10615-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UFD1L antibody (10615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HEK-293 using 10615-1-AP
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human UFD1L (10615-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

UFD1L Polyclonal antibody

UFD1L Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10615-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

UFD1, Ubiquitin recognition factor in ER-associated degradation protein 1, Ubiquitin fusion degradation protein 1, UB fusion protein 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (20)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, mouse thymus tissue, Raji cells, HepG2 cells, human testis tissue
Positive IP detected in	Raji cells
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells
Positive FC (Intra) detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 15 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

10615-1-AP targets UFD1L in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UFD1L fusion protein Ag0954 相同性解析による交差性が予測される生物種
Full Name	ubiquitin fusion degradation 1 like (yeast)
Calculated molecular weight	35 kDa
Observed molecular weight	35 kDa
GenBank accession number	BC005087
Gene Symbol	UFD1L
Gene ID (NCBI)	7353
RRID	AB_2213944
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92890
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for UFD1L antibody 10615-1-AP	Download protocol
IHC protocol for UFD1L antibody 10615-1-AP	Download protocol
IF protocol for UFD1L antibody 10615-1-AP	Download protocol
IP protocol for UFD1L antibody 10615-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IP	Science Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner. Authors - Youngdae Gwon View Article
human	WB	Nature ESCRT-III controls nuclear envelope reformation. Authors - Yolanda Olmos KD Validated View Article
human	WB	Dev Cell The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair. Authors - Samuel S Wallis KD Validated View Article
human	WB	Elife The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP Authors - Maria Körner View Article
mouse	WB	Sci Signal TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing Authors - Liangbin Lin View Article
	WB	Plant J The ubiquitin-26S proteasome system and autophagy relay proteome homeostasis regulation during silique development. Authors - Peifeng Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of K-562 using 10615-1-AP

WB analysis of HepG2 using 10615-1-AP

WB analysis of Raji using 10615-1-AP

WB analysis of human testis using 10615-1-AP

WB analysis of mouse thymus using 10615-1-AP

IP experiment of Raji using 10615-1-AP

IHC staining of human stomach using 10615-1-AP

IF Staining of HeLa using 10615-1-AP

IF Staining of HepG2 using 10615-1-AP

FC experiment of HEK-293 using 10615-1-AP

UFD1L Polyclonal antibody

UFD1L Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.

ESCRT-III controls nuclear envelope reformation.

The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.

The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP

TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing

The ubiquitin-26S proteasome system and autophagy relay proteome homeostasis regulation during silique development.