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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HEK-293 cells using UFD1L Recombinant antibody (83669-1-RR)

    FC experiment of HEK-293 using 83669-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Ufd1l Recombinant Antibody (83669-1-RR, Clone:230411A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HeLa cells using UFD1L Recombinant antibody (83669-1-RR)

    FC experiment of HeLa using 83669-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ufd1l Recombinant Antibody (83669-1-RR, Clone:230411A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83669-1-RR

    Affinity and Kinetic Characterization of 83669-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83669-1-RR against Human UFD1L were performed. The affinity constant is below 1 pM.

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UFD1L Recombinant antibody

UFD1L Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA
Cat No. 83669-1-RR
Clone No.230411A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Synonyms

UFD1, Ubiquitin recognition factor in ER-associated degradation protein 1, Ubiquitin fusion degradation protein 1, UB fusion protein 1, 230411A2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC (Intra) detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83669-1-RR targets UFD1L in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UFD1L fusion protein Ag0954 相同性解析による交差性が予測される生物種
Full Name ubiquitin fusion degradation 1 like (yeast)
Calculated molecular weight 35 kDa
GenBank accession numberBC005087
Gene Symbol UFD1L
Gene ID (NCBI) 7353
RRIDAB_3671274
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92890
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for UFD1L antibody 83669-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols