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KD/KO Validated

UGP2 Polyclonal antibody

Name: UGP2 Polyclonal antibody
Brand: Proteintech
SKU: 10391-1-AP

UGP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10391-1-AP

Synonyms

pHC379, UDP glucose pyrophosphorylase, UDPG, UDPGP, UDPGP2, UGP1, UGP2, UGPase, UGPP2

Validation Data Gallery

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AllWBIHC

WB analysis using 10391-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10391-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10391-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10391-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10391-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 10391-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10391-1-AP (UGP2 antibody) at dilution of 1:100 (under 25x lens).
IHC staining of human liver cancer using 10391-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10391-1-AP (UGP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10391-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10391-1-AP (UGP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 10391-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue, HeLa cells, HEK-293 cells, rat liver tissue
Positive IHC detected in	human ovary tumor tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 2 publications below

Product Information

10391-1-AP targets UGP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UGP2 fusion protein Ag0590 相同性解析による交差性が予測される生物種
Full Name	UDP-glucose pyrophosphorylase 2
Calculated molecular weight	56 kDa
Observed molecular weight	50-56 kDa
GenBank accession number	BC002954
Gene symbol	UGP2
Gene ID (NCBI)	7360
RRID	AB_2272775
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

UDP-glucose pyrophosphorylase 2 (UGP2, synonyms: UDPG, UGPP2, UDPGP2, Phc379)is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. So far two isoforms are encoded by two transcript variants have been found.

Protocols

Product Specific Protocols
WB protocol for UGP2 antibody 10391-1-AP	Download protocol
IHC protocol for UGP2 antibody 10391-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	iScience MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes. Authors - Dong-Xue Ding View Article
rat	IHC	Toxicol Sci Mitochondrial prohibitins and septin 9 are implicated in the onset of rat hepatocarcinogenesis. Authors - Kakehashi Anna A View Article
human	IHC	Dis Markers Low UGP2 Expression Is Associated with Tumour Progression and Predicts Poor Prognosis in Hepatocellular Carcinoma. Authors - Qiuyue Hu View Article
human	WB	J Cell Biochem Identification of UGP2 as a progression marker that promotes cell growth and motility in human glioma. Authors - Chun Zeng KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UGP2 Polyclonal antibody

UGP2 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 10391-1-AP

WB analysis of HepG2 using 10391-1-AP

WB analysis of mouse liver using 10391-1-AP

WB analysis of HEK-293 using 10391-1-AP

WB analysis of HeLa using 10391-1-AP

IHC staining of human ovary tumor using 10391-1-AP

IHC staining of human liver cancer using 10391-1-AP

IHC staining of human liver cancer using 10391-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.

Mitochondrial prohibitins and septin 9 are implicated in the onset of rat hepatocarcinogenesis.

Low UGP2 Expression Is Associated with Tumour Progression and Predicts Poor Prognosis in Hepatocellular Carcinoma.

Identification of UGP2 as a progression marker that promotes cell growth and motility in human glioma.