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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UGP2 Monoclonal antibody

UGP2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, Rat, Pig, Rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G11G6

Cat no : 68381-1-Ig

Synonyms

pHC379, UDP glucose pyrophosphorylase, UDPG, UDPGP, UDPGP2, UGP1, UGP2, UGPase, UGPP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UGP2 Monoclonal antibody (68381-1-Ig)

    WB analysis using 68381-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using UGP2 Monoclonal antibody (68381-1-Ig)

    WB analysis using 68381-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UGP2 Monoclonal antibody (68381-1-Ig)

    IHC staining of human liver cancer using 68381-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68381-1-Ig (UGP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, pig liver tissue, LNCaP cells, rabbit liver tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68381-1-Ig targets UGP2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, mouse, Rat, Pig, Rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen UGP2 fusion protein Ag33160 相同性解析による交差性が予測される生物種
Full Name UDP-glucose pyrophosphorylase 2
Calculated molecular weight 56 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC002954
Gene symbol UGP2
Gene ID (NCBI) 7360
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UDP-glucose pyrophosphorylase 2 (UGP2, synonyms: UDPG, UGPP2, UDPGP2, Phc379) is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. So far two isoforms are encoded by two transcript variants have been found.

Protocols

Product Specific Protocols
WB protocol for UGP2 antibody 68381-1-IgDownload protocol
IHC protocol for UGP2 antibody 68381-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols