Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14457-1-AP targets UGT1A10 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UGT1A10 fusion protein Ag6052 相同性解析による交差性が予測される生物種 |
| Full Name | UDP glucuronosyltransferase 1 family, polypeptide A10 |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC053576 |
| Gene Symbol | UGT1A10 |
| Gene ID (NCBI) | 54575 |
| RRID | AB_3669187 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HAW8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UGT1A10 belongs to the superfamily of UDP-glucuronosyltransferases (UGTs) catalyzing the glucuronidation reaction, which is important in the detoxification and enhanced elimination of many endogenous and exogenous substrates. Recently, UGT1A10 was the only enzyme in all 19 known human UGTs to catalyze DA glucuronidation. The UGT1As, among three subfamilies (1A, 2A, and 2B) found in human, share a common C-terminal domain of 245 residues. The N-terminal domains that determine their substrate-binding specificity are variable.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UGT1A10 antibody 14457-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |