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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UGT2B4 Polyclonal antibody

UGT2B4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15425-1-AP

Synonyms

UDPGTh 1, UDPGT 2B4, UDP-glucuronosyltransferase 2B4, Hyodeoxycholic acid-specific UDPGT, HLUG25

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using UGT2B4 Polyclonal antibody (15425-1-AP)

    WB analysis of human liver using 15425-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15425-1-AP (UGT2B4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using UGT2B4 Polyclonal antibody (15425-1-AP)

    IHC staining of human liver using 15425-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15425-1-AP (UGT2B4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using UGT2B4 Polyclonal antibody (15425-1-AP)

    IHC staining of human liver using 15425-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15425-1-AP (UGT2B4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UGT2B4 Polyclonal antibody (15425-1-AP)

    IF Staining of HepG2 using 15425-1-AP

    Immunofluorescent analysis of HepG2 cells, using UGT2B4 antibody 15425-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15425-1-AP targets UGT2B4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UGT2B4 fusion protein Ag8058 相同性解析による交差性が予測される生物種
Full Name UDP glucuronosyltransferase 2 family, polypeptide B4
Calculated molecular weight 60.5 kDa
Observed molecular weight 52-55 kDa
GenBank accession numberBC026264
Gene symbol UGT2B4
Gene ID (NCBI) 7363
RRIDAB_2214247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UGT2B4 antibody 15425-1-APDownload protocol
IHC protocol for UGT2B4 antibody 15425-1-APDownload protocol
IF protocol for UGT2B4 antibody 15425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Drug Metab Dispos

Upregulation of UGT2B4 Expression by 3'-Phosphoadenosine 5'-Phosphosulfate Synthase Knockdown: Implications for Coordinated Control of Bile Acid Conjugation.

Authors - Kathleen G Barrett
View Article