Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse thymus tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
11624-1-AP targets UHMK1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UHMK1 fusion protein Ag2204 相同性解析による交差性が予測される生物種 |
| Full Name | U2AF homology motif (UHM) kinase 1 |
| Calculated molecular weight | 419 aa, 47 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC026046 |
| Gene Symbol | UHMK1 |
| Gene ID (NCBI) | 127933 |
| RRID | AB_2214267 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TAS1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UHMK1, also named as KIS, or KIST, is a 419 amino acid protein, which contains one RRM (RNA recognition motif) domain, one protein kinase domain, and belongs to the protein kinase superfamily. UHMK1 is widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes. UHMK1 localizes in the nucleus and upon serum stimulation, phosphorylating CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. UHMK1 may be involved in trafficking and/or processing of RNA and may have a role in the developing nervous system and the adult brain.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UHMK1 antibody 11624-1-AP | Download protocol |
| IHC protocol for UHMK1 antibody 11624-1-AP | Download protocol |
| IP protocol for UHMK1 antibody 11624-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain Res Cord blood administration induces oligodendrocyte survival through alterations in gene expression. | ||
Cell Death Dis UHMK1 aids colorectal cancer cell proliferation and chemoresistance through augmenting IL-6/STAT3 signaling.
| ||
Thorac Cancer UHMK1 promotes lung adenocarcinoma oncogenesis by regulating the PI3K/AKT/mTOR signaling pathway
| ||
J Cancer Res Clin Oncol KIS, a target of SOX4, regulates the ID1-mediated enhancement of β-catenin to facilitate lung adenocarcinoma cell proliferation and metastasis
| ||
Oral Surg Oral Med Oral Pathol Oral Radiol Expression and clinical significance of U2AF homology motif kinase 1 in oral squamous cell carcinoma |