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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UHRF1 Recombinant antibody

UHRF1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241872B12

Cat no : 84489-4-RR

Synonyms

HuNp95, hUHRF1, hNp95, EC:2.3.2.27, E3 ubiquitin-protein ligase UHRF1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UHRF1 Recombinant antibody (84489-4-RR)

    WB analysis using 84489-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84489-4-RR (UHRF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UHRF1 Recombinant antibody (84489-4-RR)

    IF Staining of HepG2 using 84489-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UHRF1 antibody (84489-4-RR, Clone: 241872B12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using UHRF1 Recombinant antibody (84489-4-RR)

    FC experiment of U2OS using 84489-4-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug UHRF1 Recombinant antibody (84489-4-RR, Clone:241872B12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

Positive WB detected inMCF-7 cells, HuH-7 cells, HeLa cells, Jurkat cells, HepG2 cells, HCT-116 cells, U2OS cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84489-4-RR is UHRF1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UHRF1 fusion protein Ag17498 相同性解析による交差性が予測される生物種
Full Name ubiquitin-like with PHD and ring finger domains 1
Calculated molecular weight 806 aa, 91 kDa
Observed molecular weight100 kDa
GenBank accession numberBC113875
Gene symbol UHRF1
Gene ID (NCBI) 29128
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).

Protocols

Product Specific Protocols
WB protocol for UHRF1 antibody 84489-4-RRDownload protocol
IF protocol for UHRF1 antibody 84489-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols