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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UHRF1 Recombinant antibody (84489-5-RR)

    WB analysis using 84489-5-RR (same clone as 84489-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84489-5-RR (UHRF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84489-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UHRF1 Recombinant antibody (84489-5-RR)

    IF Staining of HepG2 using 84489-5-RR (same clone as 84489-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UHRF1 antibody (84489-5-RR, Clone: 241872G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84489-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84489-5-RR

    Affinity and Kinetic Characterization of 84489-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84489-5-RR against Human UHRF1 were performed. The affinity constant is 0.842 nM.

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UHRF1 Recombinant antibody, PBS Only

UHRF1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84489-5-PBS
Clone No.241872G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

HuNp95, hUHRF1, hNp95, EC:2.3.2.27, E3 ubiquitin-protein ligase UHRF1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84489-5-PBS targets UHRF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UHRF1 fusion protein Ag17498 相同性解析による交差性が予測される生物種
Full Name ubiquitin-like with PHD and ring finger domains 1
Calculated molecular weight 806 aa, 91 kDa
Observed molecular weight100 kDa
GenBank accession numberBC113875
Gene Symbol UHRF1
Gene ID (NCBI) 29128
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96T88
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).