• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UIMC1 Polyclonal antibody

UIMC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13642-1-AP

Synonyms

Ubiquitin interaction motif-containing protein 1, RXRIP110, Retinoid X receptor-interacting protein 110, Receptor-associated protein 80, RAP80

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse colon tissue using UIMC1 Polyclonal antibody (13642-1-AP)

    WB analysis of mouse colon using 13642-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using UIMC1 Polyclonal antibody (13642-1-AP)

    WB analysis of HeLa using 13642-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using UIMC1 Polyclonal antibody (13642-1-AP)

    WB analysis of COLO 320 using 13642-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using UIMC1 Polyclonal antibody (13642-1-AP)

    WB analysis of COLO 320 using 13642-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using UIMC1 Polyclonal antibody (13642-1-AP)

    WB analysis of Raji using 13642-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using UIMC1 Polyclonal antibody (13642-1-AP)

    WB analysis of HeLa using 13642-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using UIMC1 Polyclonal antibody (13642-1-AP)

    IP experiment of COLO 320 using 13642-1-AP

    IP result of anti-UIMC1 (IP:13642-1-AP, 4ug; Detection:13642-1-AP 1:500) with COLO 320 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using UIMC1 Polyclonal antibody (13642-1-AP)

    IHC staining of human testis using 13642-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13642-1-AP (UIMC1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using UIMC1 Polyclonal antibody (13642-1-AP)

    IHC staining of human testis using 13642-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13642-1-AP (UIMC1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UIMC1 Polyclonal antibody (13642-1-AP)

    IF Staining of HeLa using 13642-1-AP

    Immunofluorescent analysis of Hela cells, using UIMC1 antibody 13642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UIMC1 Polyclonal antibody (13642-1-AP)

    IF Staining of HeLa using 13642-1-AP

    Immunofluorescent analysis of Hela cells, using UIMC1 antibody 13642-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using UIMC1 Polyclonal antibody (13642-1-AP)

    IF Staining of MCF-7 using 13642-1-AP

    Immunofluorescent analysis of MCF-7 cells, using UIMC1 antibody 13642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inmouse colon tissue, COLO 320 cells, HeLa cells, Raji cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13642-1-AP targets UIMC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UIMC1 fusion protein Ag4582 相同性解析による交差性が予測される生物種
Full Name ubiquitin interaction motif containing 1
Calculated molecular weight 719 aa, 80 kDa
Observed molecular weight 97 kDa, 80 kDa, 71-75 kDa, 61 kDa
GenBank accession numberBC032561
Gene symbol UIMC1
Gene ID (NCBI) 51720
RRIDAB_10641052
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UIMC1 antibody 13642-1-APDownload protocol
IHC protocol for UIMC1 antibody 13642-1-APDownload protocol
IF protocol for UIMC1 antibody 13642-1-APDownload protocol
IP protocol for UIMC1 antibody 13642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols