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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ULBP2 Recombinant antibody

ULBP2 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240413A6

Cat no : 83709-1-RR

Synonyms

NKG2D ligand 2, N2DL-2, N2DL2, ALCAN-alpha, ALCAN alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ULBP2 Recombinant antibody (83709-1-RR)

    WB analysis using 83709-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83709-1-RR (ULBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ULBP2 Recombinant antibody (83709-1-RR)

    IHC staining of human colon cancer using 83709-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83709-1-RR (ULBP2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ULBP2 Recombinant antibody (83709-1-RR)

    IHC staining of human colon cancer using 83709-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83709-1-RR (ULBP2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83709-1-RR

    Affinity and Kinetic Characterization of 83709-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83709-1-RR against Human ULBP2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inJurkat cells, Saos-2 cells, THP-1 cells, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83709-1-RR is ULBP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name UL16 binding protein 2
Calculated molecular weight27 kDa
Observed molecular weight30 kDa
GenBank accession numberNM_025217.4
Gene symbol ULBP2
Gene ID (NCBI) 80328
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NKG2D is an activating cell surface receptor that is predominantly expressed on cytotoxic immune cells. NKG2D recognizes a wide range of ligands. In humans, the NKG2D ligand (NKG2DL) includes MICA, MICB, and six members of the ULBP family (ULBP1-6) (PMID: 29568297; 30813924). ULBPs are MHC class I-related molecules (PMID: 11239445). ULBP2 contains two Ig-like domains, the alpha-1 and alpha-2 domains characteristic of the MHC class I family, but lacks the alpha-3 domain. It can be expressed as either a transmembrane or GPI-linked protein, or released from the cell surface (PMID: 21224393; 24614922). ULBP2 binds and activates the NKG2D, mediating the recruitment and activation of NK cells.

Protocols

Product Specific Protocols
WB protocol for ULBP2 antibody 83709-1-RRDownload protocol
IHC protocol for ULBP2 antibody 83709-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols