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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ULBP3 Polyclonal antibody (30058-1-AP)

    WB analysis using 30058-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30058-1-AP (ULBP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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ULBP3 Polyclonal antibody

ULBP3 Polyclonal Antibody for WB, ELISA
Cat No. 30058-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

ALCAN gamma, N2DL 3, N2DL3, NKG2D ligand 3, NKG2DL3, RAET1N, UL16 binding protein 3, ULBP3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MDA-MB-231 cells, SW480 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30058-1-AP targets ULBP3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ULBP3 fusion protein Ag32424 相同性解析による交差性が予測される生物種
Full Name UL16 binding protein 3
Calculated molecular weight28 kDa
Observed molecular weight28 kDa
Gene Symbol ULBP3
Gene ID (NCBI) 79465
RRIDAB_3086222
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZM4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ULBP3 (UL16 binding protein-3), a class 1 major histocompatibility complex-like (MHC-like) molecule and member of the family of ligands of the killer cell lectin-like receptor subfamily K member 1 (NKG2D) (PMID:31292299). ULBP3 are expressed at the cell surface of multiple types of cells and tissues as a glycosylphosphatidylinositol (GPI)-linked proteins (PMID:22741585).

Protocols

Product Specific Protocols
WB protocol for ULBP3 antibody 30058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols