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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ULK1 Polyclonal antibody

ULK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27352-1-AP

Synonyms

ATG1, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ULK1 Polyclonal antibody (27352-1-AP)

    WB analysis using 27352-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ULK1 Polyclonal antibody (27352-1-AP)

    WB analysis using 27352-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ULK1 Polyclonal antibody (27352-1-AP)

    WB analysis using 27352-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ULK1 Polyclonal antibody (27352-1-AP)

    IHC staining of human liver cancer using 27352-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ULK1 Polyclonal antibody (27352-1-AP)

    IHC staining of mouse skeletal muscle using 27352-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ULK1 Polyclonal antibody (27352-1-AP)

    IHC staining of mouse skeletal muscle using 27352-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ULK1 Polyclonal antibody (27352-1-AP)

    IHC staining of human liver cancer using 27352-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ULK1 Polyclonal antibody (27352-1-AP)

    IF Staining of HepG2 using 27352-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed 48孔 HepG2 cells using ULK1 antibody (27352-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, C2C12 cells, HepG2 cells, HSC-T6 cellls
Positive IHC detected inmouse skeletal muscle tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in48孔 HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27352-1-AP targets ULK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ULK1 fusion protein Ag22397 相同性解析による交差性が予測される生物種
Full Name unc-51-like kinase 1 (C. elegans)
Observed molecular weight 140-150 kDa
GenBank accession numberNM_003565
Gene symbol ULK1
Gene ID (NCBI) 8408
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318). ULK1 is a 113 kDa protein that it also can be detected a band of 150 kDa through the western blot (PMID: 32904616, PMID: 32899862, PMID: 37270528).

Protocols

Product Specific Protocols
WB protocol for ULK1 antibody 27352-1-APDownload protocol
IHC protocol for ULK1 antibody 27352-1-APDownload protocol
IF protocol for ULK1 antibody 27352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols