Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, HEK-293T cells, COLO 320 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IHC | See 3 publications below |
| IP | See 1 publications below |
Product Information
14830-1-AP targets UMPS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UMPS fusion protein Ag6620 相同性解析による交差性が予測される生物種 |
| Full Name | uridine monophosphate synthetase |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 52 kDa, 45 kDa |
| GenBank accession number | BC000364 |
| Gene Symbol | UMPS |
| Gene ID (NCBI) | 7372 |
| RRID | AB_2212392 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11172 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UMPS, also named as OPRT and ODC, plays an important role in pyrimidine synthesis, converting orotic acid to uridine 5′ monophosphate. UMPS has four isoforms with MW 53 kDa, 43 kDa, 33 kDa and 23 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UMPS antibody 14830-1-AP | Download protocol |
| IHC protocol for UMPS antibody 14830-1-AP | Download protocol |
| IP protocol for UMPS antibody 14830-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.
| ||
Onco Targets Ther Prognostic significance of thymidylate synthase in postoperative non-small cell lung cancer patients. | ||
ESMO Open Immunohistochemical validation study of 15-gene biomarker panel predictive of benefit from adjuvant chemotherapy in resected non-small-cell lung cancer: analysis of JBR.10. | ||
Nat Commun Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia. | ||
Cell Death Differ Oxoglutarate dehydrogenase-like inhibits the progression of hepatocellular carcinoma by inducing DNA damage through non-canonical function |