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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UNC13A Monoclonal antibody (68483-1-Ig)

    WB analysis using 68483-1-Ig (same clone as 68483-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68483-1-Ig (UNC13A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68483-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using UNC13A Monoclonal antibody (68483-1-Ig)

    IHC staining of mouse brain using 68483-1-Ig (same clone as 68483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68483-1-Ig (UNC13A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68483-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using UNC13A Monoclonal antibody (68483-1-Ig)

    IHC staining of mouse brain using 68483-1-Ig (same clone as 68483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68483-1-Ig (UNC13A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68483-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using UNC13A Monoclonal antibody (68483-1-Ig)

    IF Staining of SH-SY5Y using 68483-1-Ig (same clone as 68483-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using UNC13A antibody (68483-1-Ig, Clone: 3C3A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68483-1-PBS in a different storage buffer formulation.

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UNC13A Monoclonal antibody, PBS Only

UNC13A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68483-1-PBS
Clone No.3C3A9

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA1032, Munc13 1, Protein unc 13 homolog A, unc 13 homolog A (C. elegans), UNC13A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68483-1-PBS targets UNC13A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name unc-13 homolog A (C. elegans)
Calculated molecular weight 193 kDa
Observed molecular weight193-210 kDa
GenBank accession numberNM_001080421
Gene Symbol UNC13A
Gene ID (NCBI) 23025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UPW8
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.