UNC13D/Munc13-4 Polyclonal antibody
UNC13D/Munc13-4 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 16905-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, K-562 cells, human placenta tissue |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 6 publications below |
IF | See 2 publications below |
Product Information
16905-1-AP targets UNC13D/Munc13-4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | UNC13D/Munc13-4 fusion protein Ag10519 相同性解析による交差性が予測される生物種 |
Full Name | unc-13 homolog D (C. elegans) |
Calculated molecular weight | 1090 aa, 103 kDa |
Observed molecular weight | 120 kDa |
GenBank accession number | BC067084 |
Gene symbol | UNC13D |
Gene ID (NCBI) | 201294 |
RRID | AB_2212713 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for UNC13D/Munc13-4 antibody 16905-1-AP | Download protocol |
IHC protocol for UNC13D/Munc13-4 antibody 16905-1-AP | Download protocol |
IP protocol for UNC13D/Munc13-4 antibody 16905-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells | ||
J Exp Med Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency. | ||
Blood Familial hemophagocytic lymphohistiocytosis type 3 (FHL3) caused by deep intronic mutation and inversion in UNC13D. | ||
Front Immunol Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets. | ||
Mol Biol Cell A novel Munc13-4/S100A10/Annexin A2 complex promotes Weibel-Palade body exocytosis in endothelial cells. | ||
J Transl Med Recycling machinery of integrin coupled with focal adhesion turnover via RAB11-UNC13D-FAK axis for migration of pancreatic cancer cells |