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  • KD/KO Validated

UNC45A Polyclonal antibody

UNC45A Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 19564-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Protein unc 45 homolog A, IRO039700, GCUNC-45, GCUNC45, GCUNC 45

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inK-562 cells, mouse lung tissue, mouse ovary tissue, HeLa cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19564-1-AP targets UNC45A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name unc-45 homolog A (C. elegans)
Calculated molecular weight 103 kDa
Observed molecular weight 100 kDa
GenBank accession numberNM_018671
Gene Symbol UNC45A
Gene ID (NCBI) 55898
RRIDAB_10644335
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3U1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UNC45A, also named as SMAP1, acts as co-chaperone for HSP90. UNC45A prevents the stimulation of HSP90AB1 ATPase activity by AHSA1. It is a positive factor in promoting PGR function in the cell. It is necessary for proper folding of myosin and necessary for normal cell proliferation, normal myotube formation and myosin accumulation during muscle cell development. UNC45A plays a role in erythropoiesis in stroma cells in the spleen. The antibody recognizes the C-term of UNC45A.

Protocols

Product Specific Protocols
WB protocol for UNC45A antibody 19564-1-APDownload protocol
IHC protocol for UNC45A antibody 19564-1-APDownload protocol
IP protocol for UNC45A antibody 19564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FASEB J

A myosin chaperone, UNC-45A, is a novel regulator of intestinal epithelial barrier integrity and repair.

Authors - Susana Lechuga
  • KO Validated
ratWB, IF

J Cell Sci

UNC-45A breaks the microtubule lattice independently of its effects on non-muscle myosin II.

Authors - Juri Habicht
mouseIHC,IF

Cells

UNC-45A Is Highly Expressed in the Proliferative Cells of the Mouse Genital Tract and in the Microtubule-Rich Areas of the Mouse Nervous System.

Authors - Valentino Clemente
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