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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UNC45B Polyclonal antibody

UNC45B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21640-1-AP

Synonyms

CMYA4, Protein unc 45 homolog B, SMUNC45, unc 45 homolog B (C. elegans), Unc 45B, UNC45, unc-45 myosin chaperone B, UNC45B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UNC45B Polyclonal antibody (21640-1-AP)

    WB analysis using 21640-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21640-1-AP (UNC45B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using UNC45B Polyclonal antibody (21640-1-AP)

    IHC staining of mouse heart using 21640-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21640-1-AP (UNC45B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21640-1-AP targets UNC45B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UNC45B fusion protein Ag16294 相同性解析による交差性が予測される生物種
Full Name unc-45 homolog B (C. elegans)
Calculated molecular weight 931 aa, 104 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC101063
Gene symbol UNC45B
Gene ID (NCBI) 146862
RRIDAB_10859787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UNC45B antibody 21640-1-APDownload protocol
IHC protocol for UNC45B antibody 21640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Biol Cell

The myosin chaperone UNC-45 has an important role in maintaining the structure and function of muscle sarcomeres during adult aging

Authors - Courtney J Matheny
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

PK (Verified Customer) (08-14-2024)

excellent

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: ac16
UNC45B Antibody Western Blot validation (1000 dilution) in ac16 (Cat no:21640-1-AP)