Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, PC-3 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
22068-1-AP targets UNC5A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UNC5A fusion protein Ag17155 相同性解析による交差性が予測される生物種 |
| Full Name | unc-5 homolog A (C. elegans) |
| Calculated molecular weight | 842 aa, 93 kDa |
| Observed molecular weight | 88-100 kDa |
| GenBank accession number | BC157824 |
| Gene Symbol | UNC5A |
| Gene ID (NCBI) | 90249 |
| RRID | AB_11182161 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZN44 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UNC5A, also named as KIAA1976 and UNC5H1, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UNC5A antibody 22068-1-AP | Download protocol |
| IHC protocol for UNC5A antibody 22068-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Psychiatry YTHDF2 in dentate gyrus is the m6A reader mediating m6A modification in hippocampus-dependent learning and memory | ||
Int J Clin Exp Pathol The association of UNC5A expression with the clinicopathologic features and prognosis of radiotherapy in patients with non-small-cell lung cancer. | ||
Cell Commun Signal Netrin-1 induces the anti-apoptotic and pro-survival effects of B-ALL cells through the Unc5b-MAPK axis | ||
Cancers (Basel) Upregulation of Cell Surface Glycoproteins in Correlation with KSHV LANA in the Kaposi Sarcoma Tumor Microenvironment |