UNC5C-Specific Polyclonal antibody
UNC5C-Specific Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 20240-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, mouse kidney tissue, unboiled rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
20240-1-AP targets UNC5C-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | unc-5 homolog C (C. elegans) |
| Calculated molecular weight | 103 kDa |
| Observed molecular weight | 50 kDa, 103 kDa |
| GenBank accession number | NM_003728 |
| Gene symbol | UNC5C |
| Gene ID (NCBI) | 8633 |
| RRID | AB_10695632 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UNC5C, also named as UNC5H3, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. The antibody is specific to UNC5C.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UNC5C-Specific antibody 20240-1-AP | Download protocol |
| IHC protocol for UNC5C-Specific antibody 20240-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Alcohol Clin Exp Res Fetal alcohol exposure impairs learning and memory functions and elevates levels of various biochemical markers of Alzheimer's disease in the brain of twelve-month-old rats | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Makenna (Verified Customer) (07-16-2024) | Didn't work in western blots of IF--ended up having a custom Unc5C antibody made by ProteinTech
|
FH Devi (Verified Customer) (02-01-2019) | 20ug mouse brain cortex homogenates (lane 1 and 3: WT and Lane 2: Unc5c KO) were loaded and probed with rabbit anti-Unc5c antibody at a dilution of 1:3000 with 5% milk in TBST for 1.5 hr at room temperature. Secondary antibody was used in 1:5000 dilution and developed using ECL west femto. The only concern was that KO also showed a band albeit a faint one. WT reprised the bands showedas expected although I have concerns about bands showing up in KO tissue.
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