Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse kidney tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29941-1-AP targets UNC5D in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UNC5D fusion protein Ag31973 相同性解析による交差性が予測される生物種 |
| Full Name | unc-5 homolog D (C. elegans) |
| Calculated molecular weight | 106KD |
| Observed molecular weight | 120 kDa, 68 kDa |
| GenBank accession number | NM_080872 |
| Gene Symbol | UNC5D |
| Gene ID (NCBI) | 137970 |
| RRID | AB_3086195 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UXZ4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UNC5D, also named as KIAA1777 and UNC5H4, belongs to the unc-5 family. It is a receptor for netrin. It may be involved in axon guidance by mediating axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Previous studies have demonstrated that UNC5D can be cleavaged by caspase 2 and 3, suggesting that UNC5D serves as a direct substrate for caspases 2 and 3 (PMID: 23778138).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UNC5D antibody 29941-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |