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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using UNC5D-Specific Polyclonal antibody (20241-1-AP)

    WB analysis of mouse kidney using 20241-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20241-1-AP (UNC5D-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using UNC5D-Specific Polyclonal antibody (20241-1-AP)

    WB analysis of mouse kidney using 20241-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20241-1-AP (UNC5D-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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UNC5D-Specific Polyclonal antibody

UNC5D-Specific Polyclonal Antibody for WB, ELISA
Cat No. 20241-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

KIAA1777, Netrin receptor UNC5D, PRO34692, Protein unc 5 homolog 4, Protein unc 5 homolog D, unc 5 homolog D (C. elegans), UNC5D, Unc5h4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20241-1-AP targets UNC5D-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name unc-5 homolog D (C. elegans)
Calculated molecular weight 106 kDa
Observed molecular weight 59-65 kDa
GenBank accession numberNM_080872
Gene Symbol UNC5D
Gene ID (NCBI) 137970
RRIDAB_10699878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXZ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UNC5D, also named as KIAA1777 and UNC5H4, belongs to the unc-5 family. It is a receptor for netrin. It may be involved in axon guidance by mediating axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. The antibody is specific to UNC5D.

Protocols

Product Specific Protocols
WB protocol for UNC5D-Specific antibody 20241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Sci Adv

The FLRT3-UNC5B checkpoint pathway inhibits T cell-based cancer immunotherapies

Authors - Kushal Prajapati
View Article