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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UNC93B1 Polyclonal antibody (28359-1-AP)

    WB analysis using 28359-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28359-1-AP (UNC93B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using UNC93B1 Polyclonal antibody (28359-1-AP)

    IHC staining of human ovary cancer using 28359-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28359-1-AP (UNC93B1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

UNC93B1 Polyclonal antibody

UNC93B1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28359-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

UNC93, Unc 93B1, unc 93 homolog B1 (C. elegans), Protein unc-93 homolog B1, Protein unc 93 homolog B1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue, rat kidney tissue, rat spleen tissue, pig spleen tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28359-1-AP targets UNC93B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UNC93B1 fusion protein Ag27937 相同性解析による交差性が予測される生物種
Full Name unc-93 homolog B1 (C. elegans)
Calculated molecular weight 597 aa, 67 kDa
Observed molecular weight~70 kDa
GenBank accession numberBC101568
Gene Symbol UNC93B1
Gene ID (NCBI) 81622
RRIDAB_3086046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1C4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Unc-93 homolog B1 (UNC93B1) is a key regulator of nucleic acid (NA)-sensing Toll-like receptors (TLRs). UNC93B1 plays an important role in innate and adaptive immunity by regulating nucleotide-sensing Toll-like receptor (TLR) signaling. It's a transmembrane protein that is known to control the movement of TLRs from the endoplasmic reticulum-where TLRs are assembled-to endosomes. The calculated MW of UNC93B1 is 66 kDa, 28359-1-AP can detect a band around 70 kDa.

Protocols

Product Specific Protocols
WB protocol for UNC93B1 antibody 28359-1-APDownload protocol
IHC protocol for UNC93B1 antibody 28359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols