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  • Featured Product
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UNG Monoclonal antibody

UNG Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B3B2

Cat no : 67490-1-Ig

Synonyms

DGU, DKFZp781L1143, HIGM4, UDG, UNG, UNG1, UNG15, UNG2, uracil DNA glycosylase

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using UNG Monoclonal antibody (67490-1-Ig)

    WB analysis of HepG2 using 67490-1-Ig

    WB result of UNG antibody (67490-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UNG transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using UNG Monoclonal antibody (67490-1-Ig)

    WB analysis using 67490-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67490-1-Ig (UNG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UNG Monoclonal antibody (67490-1-Ig)

    IHC staining of human liver cancer using 67490-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67490-1-Ig (UNG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UNG Monoclonal antibody (67490-1-Ig)

    IHC staining of human liver cancer using 67490-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67490-1-Ig (UNG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using UNG Monoclonal antibody (67490-1-Ig)

    IHC staining of human prostate cancer using 67490-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67490-1-Ig (UNG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells, HT-29 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67490-1-Ig targets UNG in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UNG fusion protein Ag29979 相同性解析による交差性が予測される生物種
Full Name uracil-DNA glycosylase
Calculated molecular weight 304 aa, 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC015205
Gene symbol UNG
Gene ID (NCBI) 7374
RRIDAB_2918492
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UNG antibody 67490-1-IgDownload protocol
IHC protocol for UNG antibody 67490-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols