Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, LNCaP cells, HT-29 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human liver cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67490-1-Ig targets UNG in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | UNG fusion protein Ag29979 相同性解析による交差性が予測される生物種 |
| Full Name | uracil-DNA glycosylase |
| Calculated molecular weight | 304 aa, 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC015205 |
| Gene Symbol | UNG |
| Gene ID (NCBI) | 7374 |
| RRID | AB_2918492 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P13051 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UNG antibody 67490-1-Ig | Download protocol |
| IHC protocol for UNG antibody 67490-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |