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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UNK Polyclonal antibody (31574-1-AP)

    WB analysis using 31574-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31574-1-AP (UNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using UNK Polyclonal antibody (31574-1-AP)

    IHC staining of human colon using 31574-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 31574-1-AP (UNK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using UNK Polyclonal antibody (31574-1-AP)

    IF Staining of HEK-293 using 31574-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using UNK antibody (31574-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UNK Polyclonal antibody (31574-1-AP)

    IF Staining of HeLa using 31574-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using UNK antibody (31574-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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UNK Polyclonal antibody

UNK Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31574-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ZC3HDC5, ZC3H5, unkempt homolog (Drosophila), KIAA1753

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells
Positive IF/ICC detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31574-1-AP targets UNK in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UNK fusion protein Ag36222 相同性解析による交差性が予測される生物種
Full Name unkempt homolog (Drosophila)
Observed molecular weight88~100 kDa
GenBank accession numberBC053362
Gene Symbol UNK
Gene ID (NCBI) 85451
RRIDAB_3670041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9C0B0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Unkempt (UNK) is a sequence-specific RNA-binding protein which plays an important role in the establishment and maintenance of the early morphology of cortical neurons during embryonic development. UNK gene encodes a zinc finger/RING domain containing protein, as a negative regulator of the timing of photoreceptor differentiation.

Protocols

Product Specific Protocols
WB protocol for UNK antibody 31574-1-APDownload protocol
IF protocol for UNK antibody 31574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols