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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UPB1 Polyclonal antibody

UPB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24603-1-AP

Synonyms

Beta alanine synthase, Beta ureidopropionase, BUP 1, BUP1, UPB1, ureidopropionase, beta

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using UPB1 Polyclonal antibody (24603-1-AP)

    WB analysis of mouse liver using 24603-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24603-1-AP (UPB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using UPB1 Polyclonal antibody (24603-1-AP)

    IHC staining of human heart using 24603-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24603-1-AP (UPB1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using UPB1 Polyclonal antibody (24603-1-AP)

    IHC staining of human heart using 24603-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24603-1-AP (UPB1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24603-1-AP is UPB1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UPB1 fusion protein Ag18960 相同性解析による交差性が予測される生物種
Full Name ureidopropionase, beta
Calculated molecular weight 384 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC131703
Gene symbol UPB1
Gene ID (NCBI) 51733
RRIDAB_2879634
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BUP1(Beta-ureidopropionase) is also named as BUP1, Beta-alanine synthase, N-carbamoyl-beta-alanine amidohydrolase and belongs to the CN hydrolase family. It catalyzes the last step in the pyrimidine degradation pathway that it converts N-carbamyl-beta-aminoisobutyric acid and N-carbamyl-beta-alanine to, respectively, beta-aminoisobutyric acid and beta-alanine, ammonia and carbon dioxide. It is a protein with a calculated molecular mass of 43 kDa.

Protocols

Product Specific Protocols
WB protocol for UPB1 antibody 24603-1-APDownload protocol
IHC protocol for UPB1 antibody 24603-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols