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  • Featured Product
  • KD/KO Validated

UPF1 Polyclonal antibody, PBS Only

UPF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28384-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UPF1 Polyclonal antibody (28384-1-AP)

    WB analysis using 28384-1-AP (same clone as 28384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28384-1-AP (UPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using UPF1 Polyclonal antibody (28384-1-AP)

    WB analysis of HEK-293 using 28384-1-AP (same clone as 28384-1-PBS)

    WB result of UPF1 antibody (28384-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UPF1 transfected HEK-293 cells. This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UPF1 Polyclonal antibody (28384-1-AP)

    IHC staining of human breast cancer using 28384-1-AP (same clone as 28384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28384-1-AP (UPF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UPF1 Polyclonal antibody (28384-1-AP)

    IHC staining of human breast cancer using 28384-1-AP (same clone as 28384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28384-1-AP (UPF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28384-1-PBS targets UPF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UPF1 fusion protein Ag29057 相同性解析による交差性が予測される生物種
Full Name UPF1 regulator of nonsense transcripts homolog (yeast)
Calculated molecular weight123 kDa
Observed molecular weight 123-130 kDa
GenBank accession numberBC039817
Gene symbol UPF1
Gene ID (NCBI) 5976
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.