Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55022-1-AP targets UPF2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | UPF2 regulator of nonsense transcripts homolog (yeast) |
| Calculated molecular weight | 148 kDa |
| Observed molecular weight | 148 kDa |
| GenBank accession number | NM_015542 |
| Gene Symbol | UPF2 |
| Gene ID (NCBI) | 26019 |
| RRID | AB_3086431 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HAU5 |
| Storage Buffer | 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UPF2 is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). UPF2 also plays an important role in development and cell differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UPF2 antibody 55022-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |