Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 17114-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17114-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 17114-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 17114-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17114-1-AP (UPF3A Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 17114-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17114-1-AP (UPF3A Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 17114-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17114-1-AP (UPF3A antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 17114-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17114-1-AP (UPF3A antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 17114-1-AP
Immunofluorescent analysis of HeLa cells using 17114-1-AP (UPF3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

UPF3A Polyclonal antibody

UPF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17114-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Up-frameshift suppressor 3 homolog A, UPF3, RENT3A, HUPF3A, hUpf3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, human testis tissue, Jurkat cells
Positive IHC detected in	human testis tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below

Product Information

17114-1-AP targets UPF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UPF3A fusion protein Ag10799 相同性解析による交差性が予測される生物種
Full Name	UPF3 regulator of nonsense transcripts homolog A (yeast)
Calculated molecular weight	443 aa, 51 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC023569
Gene Symbol	UPF3A
Gene ID (NCBI)	65110
RRID	AB_10694141
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H1J1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for UPF3A antibody 17114-1-AP	Download protocol
IHC protocol for UPF3A antibody 17114-1-AP	Download protocol
IF protocol for UPF3A antibody 17114-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Manag Res Functions and Clinical Significance of UPF3a Expression in Human Colorectal Cancer. Authors - Xinmin Bao KD Validated View Article
human	WB	Biochim Biophys Acta Increase of a Group of PTC(+) Transcripts by Curcumin through Inhibition of the NMD Pathway. Authors - Dairong Feng View Article
human	IHC	J Gastroenterol Hepatol Genetic compensation response could exist in colorectal cancer: UPF3A up-regulates the oncogenic homologue gene SRSF3 expression corresponding to SRSF6 to promote colorectal cancer metastasis Authors - Weimin Xu View Article
human	WB	Cells Co-Inhibition of tGLI1 and GP130 Using FDA-Approved Ketoconazole and Bazedoxifene Is Synergistic Against the Growth and Metastasis of HER2-Enriched and Triple-Negative Breast Cancers Authors - Sara Manore View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17114-1-AP

WB analysis of HeLa using 17114-1-AP

WB analysis of human testis using 17114-1-AP

IHC staining of human testis using 17114-1-AP

IHC staining of human testis using 17114-1-AP

IHC staining of human heart using 17114-1-AP

IHC staining of human heart using 17114-1-AP

IF Staining of HeLa using 17114-1-AP

UPF3A Polyclonal antibody

UPF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Functions and Clinical Significance of UPF3a Expression in Human Colorectal Cancer.

Increase of a Group of PTC(+) Transcripts by Curcumin through Inhibition of the NMD Pathway.

Genetic compensation response could exist in colorectal cancer: UPF3A up-regulates the oncogenic homologue gene SRSF3 expression corresponding to SRSF6 to promote colorectal cancer metastasis

Co-Inhibition of tGLI1 and GP130 Using FDA-Approved Ketoconazole and Bazedoxifene Is Synergistic Against the Growth and Metastasis of HER2-Enriched and Triple-Negative Breast Cancers