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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UQCC Polyclonal antibody (28114-1-AP)

    WB analysis using 28114-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28114-1-AP (UQCC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using UQCC Polyclonal antibody (28114-1-AP)

    WB analysis of HepG2 using 28114-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28114-1-AP (UQCC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using UQCC Polyclonal antibody (28114-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 28114-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28114-1-AP (UQCC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using UQCC Polyclonal antibody (28114-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 28114-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28114-1-AP (UQCC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using UQCC Polyclonal antibody (28114-1-AP)

    IHC staining of human ovary cancer using 28114-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28114-1-AP (UQCC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using UQCC Polyclonal antibody (28114-1-AP)

    IHC staining of human ovary cancer using 28114-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28114-1-AP (UQCC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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UQCC Polyclonal antibody

UQCC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28114-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CBP3, C20orf44, BZFB, bFZb, bFGF-repressed Zic-binding protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, MCF-7 cells, LNCaP cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28114-1-AP targets UQCC in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UQCC fusion protein Ag27123 相同性解析による交差性が予測される生物種
Full Name ubiquinol-cytochrome c reductase complex chaperone
Calculated molecular weight 299 aa, 35 kDa
Observed molecular weight 21-27 kDa
GenBank accession numberBC105791
Gene Symbol UQCC
Gene ID (NCBI) 55245
RRIDAB_2881065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVA1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UQCC is a transmembrane protein that is structurally similar to the mouse basic fibroblast growth factor repressed ZIC-binding protein. In mouse this protein may be involved in fibroblast growth factor regulated growth control. In humans, polymorphisms in this gene are associated with variation in human height and osteoarthritis. Alternate splicing results in multiple transcript variant.

Protocols

Product Specific Protocols
WB protocol for UQCC antibody 28114-1-APDownload protocol
IHC protocol for UQCC antibody 28114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols