Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 21705-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21705-1-AP
WB result of UQCRC1 antibody (21705-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UQCRC1 transfected HeLa cells.
WB analysis of HeLa using 21705-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.
WB analysis of human brain using 21705-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 21705-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 21705-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 21705-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse large intestine using 21705-1-AP
mouse large intestine tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 21705-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 21705-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 21705-1-AP
IP result of anti-UQCRC1 (IP:21705-1-AP, 4ug; Detection:21705-1-AP 1:500) with HeLa cells lysate 1600ug.
IHC staining of human colon using 21705-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21705-1-AP (UQCRC1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 21705-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21705-1-AP (UQCRC1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 21705-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UQCRC1 antibody (21705-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Featured Product
KD/KO Validated

UQCRC1 Polyclonal antibody

UQCRC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21705-1-AP

Publications(40)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Ubiquinol-cytochrome-c reductase complex core protein 1, QCR1, D3S3191, Cytochrome b-c1 complex subunit 1, mitochondrial, Core protein I

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (40)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, human heart tissue, mouse large intestine tissue, mouse kidney tissue, mouse spleen tissue, L02 cells, mouse liver tissue, rat liver tissue, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human normal colon, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 36 publications below
IHC	See 2 publications below
IF	See 3 publications below

Product Information

21705-1-AP targets UQCRC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UQCRC1 fusion protein Ag16322 相同性解析による交差性が予測される生物種
Full Name	ubiquinol-cytochrome c reductase core protein I
Calculated molecular weight	480 aa, 53 kDa
Observed molecular weight	45 kDa
GenBank accession number	BC009586
Gene Symbol	UQCRC1
Gene ID (NCBI)	7384
RRID	AB_10734437
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P31930
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.

Protocols

Product Specific Protocols
WB protocol for UQCRC1 antibody 21705-1-AP	Download protocol
IHC protocol for UQCRC1 antibody 21705-1-AP	Download protocol
IF protocol for UQCRC1 antibody 21705-1-AP	Download protocol
IP protocol for UQCRC1 antibody 21705-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Bull (Beijing) Targeted clearance of mitochondria by an autophagy-tethering compound (ATTEC) and its potential therapeutic effects Authors - Shuixia Tan View Article
mouse	WB	Biomaterials Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation. Authors - Xiao-Tao He View Article
human	IHC	Theranostics Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer. Authors - Qing Wang View Article
		Acta Pharmacol Sin MicroRNA-214 contributes to Ang II-induced cardiac hypertrophy by targeting SIRT3 to provoke mitochondrial malfunction. Authors - Yan-Qing Ding View Article
human	WB	Int J Nanomedicine Activation of Dynamin-Related Protein 1 and Induction of Mitochondrial Apoptosis by Exosome-Rifampicin Nanoparticles Exerts Anti-Osteosarcoma Effect Authors - Wenkai Chen View Article
human	WB	Aging (Albany NY) Celecoxib reverses the glioblastoma chemo-resistance to temozolomide through mitochondrial metabolism. Authors - Delong Yin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 21705-1-AP

WB analysis of HeLa using 21705-1-AP

WB analysis of HeLa using 21705-1-AP

WB analysis of human brain using 21705-1-AP

WB analysis of human skeletal muscle using 21705-1-AP

WB analysis of mouse skeletal muscle using 21705-1-AP

WB analysis of human heart using 21705-1-AP

WB analysis of mouse large intestine using 21705-1-AP

WB analysis of mouse kidney using 21705-1-AP

WB analysis of mouse spleen using 21705-1-AP

IP experiment of HeLa using 21705-1-AP

IHC staining of human colon using 21705-1-AP

IHC staining of human colon using 21705-1-AP

IF Staining of HepG2 using 21705-1-AP

UQCRC1 Polyclonal antibody

UQCRC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeted clearance of mitochondria by an autophagy-tethering compound (ATTEC) and its potential therapeutic effects

Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation.

Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer.

MicroRNA-214 contributes to Ang II-induced cardiac hypertrophy by targeting SIRT3 to provoke mitochondrial malfunction.

Activation of Dynamin-Related Protein 1 and Induction of Mitochondrial Apoptosis by Exosome-Rifampicin Nanoparticles Exerts Anti-Osteosarcoma Effect

Celecoxib reverses the glioblastoma chemo-resistance to temozolomide through mitochondrial metabolism.