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  • Featured Product
  • KD/KO Validated

UQCRC2 Polyclonal antibody, PBS Only

UQCRC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14742-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UQCRC2 Polyclonal antibody (14742-1-AP)

    WB analysis using 14742-1-AP (same clone as 14742-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14742-1-AP (UQCRC2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using UQCRC2 Polyclonal antibody (14742-1-AP)

    WB analysis of human colon using 14742-1-AP (same clone as 14742-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 14742-1-AP (UQCRC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using UQCRC2 Polyclonal antibody (14742-1-AP)

    IP experiment of HeLa using 14742-1-AP (same clone as 14742-1-PBS)

    IP result of anti-UQCRC2 (IP:14742-1-AP, 4ug; Detection:14742-1-AP 1:40000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UQCRC2 Polyclonal antibody (14742-1-AP)

    IHC staining of human colon cancer using 14742-1-AP (same clone as 14742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14742-1-AP (UQCRC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UQCRC2 Polyclonal antibody (14742-1-AP)

    IHC staining of human colon cancer using 14742-1-AP (same clone as 14742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14742-1-AP (UQCRC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using UQCRC2 Polyclonal antibody (14742-1-AP)

    IHC staining of human colon using 14742-1-AP (same clone as 14742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14742-1-AP (UQCRC2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UQCRC2 Polyclonal antibody (14742-1-AP)

    IF Staining of HepG2 using 14742-1-AP (same clone as 14742-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14742-1-AP (UQCRC2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L); Phalloidin stains F-actin (Red). This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UQCRC2 Polyclonal antibody (14742-1-AP)

    IF Staining of HepG2 using 14742-1-AP (same clone as 14742-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UQCRC2 antibody (14742-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UQCRC2 Polyclonal antibody (14742-1-AP)

    IF Staining of HepG2 using 14742-1-AP (same clone as 14742-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14742-1-AP (UQCRC2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14742-1-PBS targets UQCRC2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UQCRC2 fusion protein Ag6432 相同性解析による交差性が予測される生物種
Full Name ubiquinol-cytochrome c reductase core protein II
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000484
Gene symbol UQCRC2
Gene ID (NCBI) 7385
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.