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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UQCRC2 Polyclonal antibody (14742-1-AP)

    WB analysis using 14742-1-AP (same clone as 14742-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14742-1-AP (UQCRC2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using UQCRC2 Polyclonal antibody (14742-1-AP)

    WB analysis of human colon using 14742-1-AP (same clone as 14742-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 14742-1-AP (UQCRC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using UQCRC2 Polyclonal antibody (14742-1-AP)

    IP experiment of HeLa using 14742-1-AP (same clone as 14742-1-PBS)

    IP result of anti-UQCRC2 (IP:14742-1-AP, 4ug; Detection:14742-1-AP 1:40000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UQCRC2 Polyclonal antibody (14742-1-AP)

    IHC staining of human colon cancer using 14742-1-AP (same clone as 14742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14742-1-AP (UQCRC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UQCRC2 Polyclonal antibody (14742-1-AP)

    IHC staining of human colon cancer using 14742-1-AP (same clone as 14742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14742-1-AP (UQCRC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using UQCRC2 Polyclonal antibody (14742-1-AP)

    IHC staining of human colon using 14742-1-AP (same clone as 14742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14742-1-AP (UQCRC2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UQCRC2 Polyclonal antibody (14742-1-AP)

    IF Staining of HepG2 using 14742-1-AP (same clone as 14742-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14742-1-AP (UQCRC2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L); Phalloidin stains F-actin (Red). This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UQCRC2 Polyclonal antibody (14742-1-AP)

    IF Staining of HepG2 using 14742-1-AP (same clone as 14742-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UQCRC2 antibody (14742-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UQCRC2 Polyclonal antibody (14742-1-AP)

    IF Staining of HepG2 using 14742-1-AP (same clone as 14742-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14742-1-AP (UQCRC2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14742-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

UQCRC2 Polyclonal antibody, PBS Only

UQCRC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14742-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14742-1-PBS targets UQCRC2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UQCRC2 fusion protein Ag6432 相同性解析による交差性が予測される生物種
Full Name ubiquinol-cytochrome c reductase core protein II
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000484
Gene Symbol UQCRC2
Gene ID (NCBI) 7385
RRIDAB_2241442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22695
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.