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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

URG4 Monoclonal antibody, PBS Only

URG4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D5F12

Cat no : 67144-1-PBS

Synonyms

KIAA1507, up regulated gene 4, URG4, URGCP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using URG4 Monoclonal antibody (67144-1-Ig)

    WB analysis using 67144-1-Ig (same clone as 67144-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67144-1-Ig (URG4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using URG4 Monoclonal antibody (67144-1-Ig)

    IHC staining of human breast cancer using 67144-1-Ig (same clone as 67144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using URG4 Monoclonal antibody (67144-1-Ig)

    IHC staining of human breast cancer using 67144-1-Ig (same clone as 67144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using URG4 Monoclonal antibody (67144-1-Ig)

    IHC staining of human liver cancer using 67144-1-Ig (same clone as 67144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using URG4 Monoclonal antibody (67144-1-Ig)

    IHC staining of human liver cancer using 67144-1-Ig (same clone as 67144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human nasopharyngeal carcinoma tissue using URG4 Monoclonal antibody (67144-1-Ig)

    IHC staining of human nasopharyngeal carcinoma using 67144-1-Ig (same clone as 67144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 67144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human nasopharyngeal carcinoma tissue using URG4 Monoclonal antibody (67144-1-Ig)

    IHC staining of human nasopharyngeal carcinoma using 67144-1-Ig (same clone as 67144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 67144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using URG4 Monoclonal antibody (67144-1-Ig)

    IHC staining of human liver cancer using 67144-1-Ig (same clone as 67144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using URG4 Monoclonal antibody (67144-1-Ig)

    IHC staining of human liver cancer using 67144-1-Ig (same clone as 67144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67144-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using URG4 Monoclonal antibody (67144-1-Ig)

    IF Staining of HeLa using 67144-1-Ig (same clone as 67144-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using URG4 antibody (67144-1-Ig, Clone: 2D5F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67144-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67144-1-PBS targets URG4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen URG4 fusion protein Ag28635 相同性解析による交差性が予測される生物種
Full Name up-regulated gene 4
Calculated molecular weight 922 aa, 104 kDa
Observed molecular weight 104 kDa
GenBank accession numberBC018426
Gene symbol URG4
Gene ID (NCBI) 55665
RRIDAB_2882443
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence.