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  • Featured Product
  • KD/KO Validated

URM1 Polyclonal antibody

URM1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15285-1-AP

Synonyms

C9orf74, URM1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis using 15285-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis of human placenta using 15285-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis of HepG2 using 15285-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis of A549 using 15285-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis of HepG2 using 15285-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis of mouse liver using 15285-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis of A549 using 15285-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using URM1 Polyclonal antibody (15285-1-AP)

    IP experiment of HepG2 using 15285-1-AP

    IP result of anti-URM1 (IP:15285-1-AP, 4ug; Detection:15285-1-AP 1:500) with HepG2 cells lysate 3360 ug.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using URM1 Polyclonal antibody (15285-1-AP)

    IHC staining of human hepatocirrhosis using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using URM1 Polyclonal antibody (15285-1-AP)

    IHC staining of mouse liver using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15285-1-AP (URM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using URM1 Polyclonal antibody (15285-1-AP)

    IHC staining of mouse liver using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15285-1-AP (URM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using URM1 Polyclonal antibody (15285-1-AP)

    IHC staining of human hepatocirrhosis using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using URM1 Polyclonal antibody (15285-1-AP)

    IHC staining of human liver using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using URM1 Polyclonal antibody (15285-1-AP)

    IHC staining of human liver using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells, mouse liver tissue, human placenta tissue
Positive IP detected inHepG2 cells
Positive IHC detected inmouse liver tissue, human hepatocirrhosis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15285-1-AP targets URM1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen URM1 fusion protein Ag7448 相同性解析による交差性が予測される生物種
Full Name ubiquitin related modifier 1 homolog (S. cerevisiae)
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC003581
Gene symbol URM1
Gene ID (NCBI) 81605
RRIDAB_2213808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for URM1 antibody 15285-1-APDownload protocol
IHC protocol for URM1 antibody 15285-1-APDownload protocol
IP protocol for URM1 antibody 15285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Biol

Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival.

Authors - Fu Dragony D
  • KD Validated
View Article
humanWB

Hum Mol Genet

Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.

Authors - Ki Soon Kim
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ana Paula (Verified Customer) (08-03-2022)

mESC derived motor neurons 1:1000 primary ab in blocking buffer ON at 4 C 1:10000 secondary ab in TBS 1h at RT

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mESC derived motor neurons
URM1 Antibody Western Blot validation (1:1000 dilution) in mESC derived motor neurons (Cat no:15285-1-AP)