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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UROC1 Polyclonal antibody

UROC1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25032-1-AP

Synonyms

HMFN0320, UROC1, Urocanase, urocanase domain containing 1, Urocanate hydratase

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using UROC1 Polyclonal antibody (25032-1-AP)

    WB analysis of mouse liver using 25032-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25032-1-AP (UROC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using UROC1 Polyclonal antibody (25032-1-AP)

    IP experiment of mouse liver using 25032-1-AP

    IP result of anti-UROC1 (IP:25032-1-AP, 4ug; Detection:25032-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25032-1-AP is UROC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UROC1 fusion protein Ag18143 相同性解析による交差性が予測される生物種
Full Name urocanase domain containing 1
Calculated molecular weight 676 aa, 75 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC115405
Gene symbol UROC1
Gene ID (NCBI) 131669
RRIDAB_2879859
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UROC1, also named as Urocanase and Imidazolonepropionate hydrolase, is involved in Urocanase deficiency (UROCD). UROC1 has some isoforms with MW 65-75 kDa and 80 kDa.

Protocols

Product Specific Protocols
WB protocol for UROC1 antibody 25032-1-APDownload protocol
IP protocol for UROC1 antibody 25032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols