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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UROD Polyclonal antibody (15547-1-AP)

    WB analysis using 15547-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using UROD Polyclonal antibody (15547-1-AP)

    WB analysis of HepG2 using 15547-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using UROD Polyclonal antibody (15547-1-AP)

    WB analysis of HEK-293 using 15547-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using UROD Polyclonal antibody (15547-1-AP)

    WB analysis of rat kidney using 15547-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using UROD Polyclonal antibody (15547-1-AP)

    IP experiment of K-562 using 15547-1-AP

    IP result of anti-UROD (IP:15547-1-AP, 4ug; Detection:15547-1-AP 1:500) with K-562 cells lysate 4400 ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using UROD Polyclonal antibody (15547-1-AP)

    IHC staining of human kidney using 15547-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15547-1-AP (UROD antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using UROD Polyclonal antibody (15547-1-AP)

    IHC staining of human kidney using 15547-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15547-1-AP (UROD antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using UROD Polyclonal antibody (15547-1-AP)

    IF Staining of HEK-293 using 15547-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using UROD antibody (15547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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UROD Polyclonal antibody

UROD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15547-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

URO-D, EC:4.1.1.37

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HepG2 cells, HEK-293 cells, rat kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15547-1-AP targets UROD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UROD fusion protein Ag7905 相同性解析による交差性が予測される生物種
Full Name uroporphyrinogen decarboxylase
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC001778
Gene Symbol UROD
Gene ID (NCBI) 7389
RRIDAB_2213813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06132
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UROD antibody 15547-1-APDownload protocol
IHC protocol for UROD antibody 15547-1-APDownload protocol
IF protocol for UROD antibody 15547-1-APDownload protocol
IP protocol for UROD antibody 15547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability.

Authors - Gokhan Unlu
View Article
mouseWB

Cell Mol Gastroenterol Hepatol

The histamine pathway is a target to treat hepatic experimental erythropoietic protoporphyria

Authors - Ning Kuo
View Article