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USE1 Polyclonal antibody
USE1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25218-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
The immunogen of 25218-1-AP is USE1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | USE1 fusion protein Ag18587 相同性解析による交差性が予測される生物種 |
| Full Name | unconventional SNARE in the ER 1 homolog (S. cerevisiae) |
| Calculated molecular weight | 259 aa, 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC008455 |
| Gene symbol | USE1 |
| Gene ID (NCBI) | 55850 |
| RRID | AB_2879966 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USE1(Vesicle transport protein USE1) is also named as USE1L, p31 and belongs to the USE1 family. It may be an essential molecule involved in the maintenance of ER morphology (and its deficiency leads to ER stress-induced apoptosis). USE1 also participates in the degradative function of lysosomes. This protein has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USE1 antibody 25218-1-AP | Download protocol |
| IHC protocol for USE1 antibody 25218-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Pathog SNARE protein USE1 is involved in the glycosylation and the expression of mumps virus fusion protein and important for viral propagation
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