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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis using 67114-1-Ig (same clone as 67114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis of HeLa using 67114-1-Ig (same clone as 67114-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis of HEK-293 using 67114-1-Ig (same clone as 67114-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis of HT-29 using 67114-1-Ig (same clone as 67114-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis of K-562 using 67114-1-Ig (same clone as 67114-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis of HSC-T6 using 67114-1-Ig (same clone as 67114-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis of NIH/3T3 using 67114-1-Ig (same clone as 67114-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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USF1 Monoclonal antibody, PBS Only

USF1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67114-1-PBS
Clone No.1C8F1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67114-1-PBS targets USF1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USF1 fusion protein Ag17785 相同性解析による交差性が予測される生物種
Full Name upstream transcription factor 1
Calculated molecular weight 34 kDa
Observed molecular weight 38-43 kDa
GenBank accession numberBC035505
Gene Symbol USF1
Gene ID (NCBI) 7391
RRIDAB_2882418
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22415
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa