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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Harmonin Polyclonal antibody (11358-1-AP)

    WB analysis using 11358-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11358-1-AP (Harmonin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Harmonin Polyclonal antibody (11358-1-AP)

    WB analysis of HEK-293 using 11358-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11358-1-AP (Harmonin antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Harmonin Polyclonal antibody (11358-1-AP)

    IP experiment of HEK-293 using 11358-1-AP

    IP result of anti-Harmonin (IP:11358-1-AP, 4ug; Detection:11358-1-AP 1:600) with HEK-293 cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of Human Colon cancer tissue using Harmonin Polyclonal antibody (11358-1-AP)

    IHC staining of Human Colon cancer using 11358-1-AP

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human Colon cancer tissue using Harmonin Polyclonal antibody (11358-1-AP)

    IHC staining of Human Colon cancer using 11358-1-AP

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human Colon cancer tissue using Harmonin Polyclonal antibody (11358-1-AP)

    IHC staining of Human Colon cancer using 11358-1-AP

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human Colon cancer tissue using Harmonin Polyclonal antibody (11358-1-AP)

    IHC staining of Human Colon cancer using 11358-1-AP

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human Colon cancer tissue using Harmonin Polyclonal antibody (11358-1-AP)

    IHC staining of Human Colon cancer using 11358-1-AP

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Harmonin Polyclonal antibody (11358-1-AP)

    IF Staining of HepG2 using 11358-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Harmonin antibody (11358-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Harmonin Polyclonal antibody

Harmonin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11358-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

USH1C, Renal carcinoma antigen NY-REN-3, Protein PDZ-73, Autoimmune enteropathy-related antigen AIE-75, Antigen NY-CO-38/NY-CO-37

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells, mouse brain tissues, rat brain tissues
Positive IP detected inHEK-293 cells
Positive IHC detected inHuman Colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11358-1-AP targets Harmonin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Harmonin fusion protein Ag1919 相同性解析による交差性が予測される生物種
Full Name Usher syndrome 1C (autosomal recessive, severe)
Calculated molecular weight 552 aa, 62 kDa
Observed molecular weight 68-73 kDa
GenBank accession numberBC016057
Gene Symbol USH1C/Harmonin
Gene ID (NCBI) 10083
RRIDAB_2214137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6N9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Harmonin, also known as USH1C, is a scaffold protein which interacts with other USH1 proteins (USH1B/myosin VIIa and USH1G/sans) to form complex and is implicated in proper development, morphogenesis, and structural integrity of the hair cell mechanoreceptor. Mutations of Harmonin cause Usher syndrome type 1 (congenital deafness and balance disorder, and early-onset sight loss).

Protocols

Product Specific Protocols
WB protocol for Harmonin antibody 11358-1-APDownload protocol
IHC protocol for Harmonin antibody 11358-1-APDownload protocol
IF protocol for Harmonin antibody 11358-1-APDownload protocol
IP protocol for Harmonin antibody 11358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Hum Mol Genet

Impact of the Usher syndrome on olfaction.

Authors - Fabian Jansen
View Article
humanIHC

Int J Mol Sci

A Liquid-Liquid Phase Separation-Related Index Associate with Biochemical Recurrence and Tumor Immune Environment of Prostate Cancer Patients

Authors - Qi You
View Article